Neal R. Chamberlain, PhD



Work: 660.626.2474
Fax: 660.626.2523
Email Address: nchamberlain@atsu.edu

Research Interests: Dr. Chamberlain's research involves the characterization of gene expression by Staphylococcus aureus and Staphylococcus epidermidis during growth in a biofilm. His research also aims to provide a better understanding of how bacteria in a biofilm survive following treatment with antimicrobial agents.

Education:

PhD - (Medical Microbiology and Immunology): The Ohio State University - Columbus (1985)
BS - (Biology): Indiana University of Pennsylvania (1980)
Postdoctoral Fellowship - Department of Microbiology, University of Texas Southwestern Medical School at Dallas (Dr. Michael Norgard)

Scholarly Activity

Research Publications:

2018 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW. 2018. Physician-Mentored Patient Rounds to Observe and Assess Entrustable Professional Activities 1 and 2 in Preclinical Medical Students. J. Am. Osteopath. Assoc. 118(3):199–206. doi: 10.7556/jaoa.2018.039.

2016 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW. Resources utilized by preclinical medical students during patient morning rounds. Med. Sci. Educ. 2016. 26(3):283-86. DOI:10.1007/s40670-016-0279-y.

2016 | Springer MT [student], Singh VK, Cheung AL, Donegan NP, Chamberlain NR. Effect of clpP and clpC deletion on persister cell number in Staphylococcus aureus. J Med Microbiol. 2016 Aug;65(8):848-57. doi: 10.1099/jmm.0.000304.

2016 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW. Use of large group patient rounds to characterize pre-clerkship medical students’ ability to perform three entrustable professional activities. Med. Sci. Educ. 2016. 26(3):481-89. DOI: 10.1007/s40670-016-0265-4

2012 | Chamberlain NR, Stuart MK, Singh VK, Sargentini NJ. Utilization of case presentations in medical microbiology to enhance relevance of basic science for medical students. Med Educ Online. 2012;17. doi: 10.3402/meo.v17i0.15943. Epub 2012 Mar 13

2011 | Draper BB, Johnson JC, Fossum C, Chamberlain NR. Osteopathic medical students' beliefs about osteopathic manipulative treatment at 4 colleges of osteopathic medicine. J Am Osteopath Assoc. 2011 Nov;111(11):615-30

2011 | Chamberlain NR, Singh VK. Prevalence of methicillin resistant Staphylococcus aureus in nasal samples from preclinical second-year medical students. Mo Med. 2011 Sep-Oct;108(5):373-6.

2011 | Shapiro JA, Nguyen VL, Chamberlain NR. Evidence for persisters in Staphylococcus epidermidis RP62a planktonic cultures and biofilms. J Med Microbiol. 2011 Jul;60(Pt 7):950-60. Epub 2011 Mar 17

2008 | Singh VK, Hattangady DS, Giotis ES, Singh AK, Chamberlain NR, Stuart MK, Wilkinson BJ. Insertional inactivation of branched-chain {alpha}-keto acid dehydrogenase (BKD) in Staphylococcus aureus leads to decreased branched-chain membrane fatty acids content and increased susceptibility to certain stresses. Appl Environ Microbiol. 2008 Oct;74(19):5882-5890. Epub 2008 Aug. 8

2007 | Singh VK, Utaida S, Jackson LS, Jayaswal RK, Wilkinson BJ, Chamberlain NR. Role for dnaK locus in tolerance of multiple stresses in Staphylococcus aureus. Microbiology. 2007 Sep;153(Pt 9):3162-73

2003 | Chamberlain NR, Yates HA. A prospective study of osteopathic medical students' attitudes toward use of osteopathic manipulative treatment in caring for patients. J Am Osteopath Assoc 2003 Oct;103(10):470-8

2003 | Stuart MK, Chamberlain NR. Monoclonal antibodies to elongation factor-1alpha inhibit in vitro translation in lysates of Sf21 cells. Arch Insect Biochem Physiol. 2003 Jan;52(1):17-34

2000 | Chamberlain NR, Yates HA. Use of a computer-assisted clinical case (CACC) SOAP note exercise to assess students' application of osteopathic principles and practice. J Am Osteopath Assoc 2000 Jul;100(7):437-40

1999 | Chamberlain NR. Identification and partial characterisation of an extracellular activator of fatty acid modifying enzyme (FAME) expression in Staphylococcus epidermidis. J Med Microbiol 1999 Mar;48(3):245-52

1998 | Baer RW, Chamberlain NR. Environmental factors promoting the effective use of a computer-assisted clinical case for second-year osteopathic medical students. J Am Osteopath Assoc 1998 Jul;98(7):380-5

1997 | Chamberlain NR, Brueggemann SA. Characterisation and expression of fatty acid modifying enzyme produced by Staphylococcus epidermidis. J Med Microbiol 1997 Aug;46(8):693-7

1996 | Chamberlain NR, Imanoel B. Genetic regulation of fatty acid modifying enzyme from Staphylococcus aureus. J Med Microbiol 1996 Feb;44(2):125-9

1993 | Xiong Z, Ge S, Chamberlain NR, Kapral FA. Growth cycle-induced changes in sensitivity of Staphylococcus aureus to bactericidal lipids from abscesses. J Med Microbiol 1993 Jul;39(1):58-63

1992 | Chamberlain NR. Another easy technique for extruding polyacrylamide gels from isoelectric focusing tubes of 1.5-mm inside diameter. Biotechniques 1992 Oct;13(4):532

1991 | Chamberlain NR, Mehrtens BG, Xiong Z, Kapral FA, Boardman JL, Rearick JI. Correlation of carotenoid production, decreased membrane fluidity, and resistance to oleic acid killing in Staphylococcus aureus 18Z. Infect Immun 1991 Dec;59(12):4332-7

1989 | Purcell BK, Chamberlain NR, Goldberg MS, Andrews LP, Robinson EJ, Norgard MV, Radolf JD. Molecular cloning and characterization of the 15-kilodalton major immunogen of Treponema pallidum. Infect Immun 1989 Dec;57(12):3708-14

1989 | Chamberlain NR, DeOgny L, Slaughter C, Radolf JD, Norgard MV. Acylation of the 47-kilodalton major membrane immunogen of Treponema pallidum determines its hydrophobicity. Infect Immun 1989 Sep;57(9):2878-85

1989 | Chamberlain NR, Brandt ME, Erwin AL, Radolf JD, Norgard MV. Major integral membrane protein immunogens of Treponema pallidum are proteolipids. Infect Immun 1989 Sep;57(9):2872-7

1989 | Hsu PL, Chamberlain NR, Orth K, Moomaw CR, Zhang LQ, Slaughter CA, Radolf JD, Sell S, Norgard MV. Sequence analysis of the 47-kilodalton major integral membrane immunogen of Treponema pallidum. Infect Immun 1989 Jan;57(1):196-203

1988 | Hansen EJ, Gonzales FR, Chamberlain NR, Norgard MV, Miller EE, Cope LD, Pelzel SE, Gaddy B, Clausell A. Cloning of the gene encoding the major outer membrane protein of Haemophilus influenzae type b. Infect Immun 1988 Oct;56(10):2709-16

1988 | Radolf JD, Chamberlain NR, Clausell A, Norgard MV. Identification and localization of integral membrane proteins of virulent Treponema pallidum subsp. pallidum by phase partitioning with the nonionic detergent triton X-114. Infect Immun 1988 Feb;56(2):490-8

1988 | Chamberlain NR, Radolf JD, Hsu PL, Sell S, Norgard MV. Genetic and physicochemical characterization of the recombinant DNA-derived 47-kilodalton surface immunogen of Treponema pallidum subsp. pallidum. Infect Immun 1988 Jan;56(1):71-8

1986 | Norgard MV, Chamberlain NR, Swancutt MA, Goldberg MS. Cloning and expression of the major 47-kilodalton surface immunogen of Treponema pallidum in Escherichia coli. Infect Immun 1986 Nov;54(2):500-6

1985 | Chamberlain NR (Ph.D. Thesis). The presence of delta toxin and lipase in murine intraperitoneal abscesses generated by Staphylococcus aureus. Ohio State University. 1985

2000 | Chamberlain NR. 2000. Gram Stain of Bacillus subtilis. Visual Resources: MicrobeLibrary.org

Presentations and Published Abstracts:

2019 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW, Use of Physician Mentored Patient Rounds to Observe EPA 4 (order prescriptions) in Second Year Medical Students. The AACOM Annual Conference, Washington DC. April 10-12, 2019

2017 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW. Creation of Physician-Mentored Patient Rounds (PMPR) to Observe Pre-Clinical Medical Students’ Ability to Perform Entrustable Professional Activities 1-4. Poster presented at the 3rd annual ATSU Assessment Week. February 21-23, 2017. Kirksville, MO.

2017 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW. Use of Physician-Mentored Patient Rounds (PMPR) to Observe Pre-Clinical Medical Students’ Ability to Perform Entrustable Professional Activities 1-4. Poster presented at the 3rd annual ATSU Assessment Week. February 21-23, 2017. Kirksville, MO.

2016 | Loveland AL [student], Singh VK, Chamberlain NR. Characterization of dnaK Mutation in Persistence of Staphylococcus aureus. Poster presented at the American Society for Microbiology Microbe 2016 meeting, Boston, MA. June 16-20, 2016.

2016 | Sargentini NJ, Chamberlain NR, Hudman DA (staff)), Phillips PL, Singh VK, Stuart MK. Assessing Medical Student Critical Thinking and Interpersonal Skills along with Medical Microbiology, Immunology and Infectious Diseases Knowledge. Poster presented at Assessment Week; A.T. Still University of Health Sciences; Kirksville, MO; February 9, 2016.

2016 | Stuart MK [invited speaker], Chamberlain NR, Singh VK, Hudman DA [staff], Phillips PL, Sargentini NJ. The Small-Group Clinical Case Presentation: An Instrument for Assessing Interpersonal Skills, Critical Thinking, and Knowledge in Medical Microbiology, Immunology, and Infectious Diseases. Oral presentation at the 16th Educational Strategies Workshop; Palm Springs, CA; May 15-18, 2016.

2015 | Chamberlain NR, Hardee MR, Sexton P, Baer RW. Patient Rounds to Assess Medical Students’ Critical Thinking and Clinical Reasoning. Poster presented at Assessment Week; A. T. Still University of Health Sciences; Apr. 13-16, 2015.

2015 | Chamberlain NR, Hardee MR, Sexton P, Baer RW. Utilizing Patient Rounds to Observe Medical Students’ Clinical Reasoning. Joint AACOM and AODME Annual Conference, Ft. Lauderdale, FL. Apr. 22-25, 2015.

2015 | Loveland AL [student], Singh VK, Chamberlain NR. Exploring the Effect of dnaK Mutation on Persistence in Staphylococcus aureus. Poster presented at the 7th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 31, 2015.

2015 | Sargentini NJ, Chamberlain NR, Hudman DA (Staff), Phillips PL, Singh VK, Stuart MK. Assessing Critical Thinking and Interpersonal Skills along with Medical Microbiology, Immunology and Infectious Diseases Knowledge. Poster presented at Assessment Week; A. T. Still University of Health Sciences; April 13-16, 2015.

2014 | Habtu DY [student], Chamberlain NR. Investigation into Induction of Persistence in Staphylococcus aureus SH1000. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2014 | Ricana CL [student], Hart RG [student], Chamberlain NR. Characterizing Persistence Inducing Factor in Staphylococcus epidermidis RP62A. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2013 | Chamberlain NR, Stuart MK, Singh VK, Sargentini NJ. Utilization of Clinical Case Presentations in Medical Microbiology and Infectious Diseases to Improve Student Performance on Course Assessments. Poster presented at Annual Meeting of the American Association of Colleges of Osteopathic Medicine; Baltimore, MD; April 24, 2013.

2013 | Springer MT [student], Chamberlain NR. Effect of the ClpPC Protease System in the Formation of Persister Cells in Staphylococcus aureus. Poster and oral presentation at 5th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 2, 2013.

2013 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ. Case Presentation Exercises in Immunology Enhance Relevance and Improve Student Satisfaction with the Course. Poster presented at American Society for Microbiology, 113th General Meeting; Denver, CO; May 18-21, 2013.

2012 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ. Implementation of a Small Group Clinical Case Presentation Exercise in Immunology. AMSMIC 14th Educational Strategies Workshop; Santa Fe, NM. April 28-May 2, 2012.

2011 | Budine TE [student], Chamberlain NR, Singh VK, Walston TD. C. elegans as a Model for S. aureus Virulence. Poster presented at 3rd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 22, 2011.

2011 | Draper BA [student], Johnson JC, Chamberlain NR. What Drew You to Osteopathic Medicine? A Survey of Osteopathic Medical Students from 10 Schools. Presented at: 55th Annual American Osteopathic Association Research Conference; Orlando, FL; October 30-November 1, 2011.

2010 | Budine TE [student], Chamberlain N, Singh V, Walston TD. C. elegans as a Model for S. aureus Virulence. Poster presented at 2nd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 9, 2010.

2010 | Draper BA [student], Johnson JC, Fossum C, Chamberlain NR. Assessing Medical Student Beliefs about Osteopathic Manipulative Treatment at Four Osteopathic Medical Schools. The Annual Meeting of the American Association of Colleges of Osteopathic Medicine; North Bethesda, MD; April 7-10, 2010.

2009 | Shapiro JA [student], Nguyen V [student], Chamberlain, NR; Survival of planktonic and sessile Staphylococcus epidermidis cultures treated with levofloxacin; Presented at the 1st Annual Interdisciplinary Biomedical Research Symposium; A.T. Still University of Health Sciences; Kirksville, MO; Sept. 26, 2009.

2009 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ; Microbiology for Medical Students: A Case Presentations Model; 109th General Meeting of the American Society for Microbiology; Philadelphia, PA; May 17-21, 2009.

2008 | Singh VK, Hattangady D, Giotis E, Chamberlain NR, Stuart MK, Wilkinson B; Insertional Inactivation of Branched-chain Alpha-keto Acid Dehydrogenase (BKD) in Staphylococcus aureus Leads to Decreased Branched-chain Membrane Fatty Acids Content and Increased Susceptibility to Certain Stresses; International Symposium on Staphylococci and Staphylococcal Infections; Cairns, Australia; Sept. 7 - 10, 2008.

2007 | Sargentini NJ, McNabb JE, Higbee D, Stuart MK, Chamberlain NR, Lockwood MD; Using Standardized Patient Encounters To Improve Basic Science Understanding And Integrate Professional Behavior And Clinical Skills Assessment Within Two Medical School Basic Science Courses; Central Group on Educational Affairs, Association of American Medical Colleges Spring Conference; Indianapolis, IN; March 22-24, 2007, poster presentation.

2006 | Harpenau CM, Inoue A, Johnson JC, Chamberlain NR, Jadoon-Khamash E, Noll DR; The Immediate Effects of the Splenic Pump Technique on Blood Cell Counts in Normal Adults; Journal of the American Osteopathic Association 106(8):474, 2006; American Osteopathic Association Annual Convention & Scientific Seminar; Las Vegas, NV; October 15-19, 2006.

2006 | Inoue A, Harpenau CM, Chamberlain NR, Johnson JC, Jadoon-Khamash E, Noll DR;The Immediate Effects of the Splenic Pump on Serum Level of C-reactive Protein in Normal Adults; Journal of the American Osteopathic Association 106(8):474-475, 2006; American Osteopathic Association Annual Convention & Scientific Seminar; Las Vegas, NV; October 15-19, 2006.

2004 | Chamberlain NR, Smart JI, Geisbuhler TP. Purification and Characterization of a Bioactive Pheromone from Staphylococcus epidermidis. The 104th General Meeting of the American Society for Microbiology; New Orleans, LA; May 23-27, 2004.

2002 | Stuart MK, Chamberlain NR. 2002. Immunological Inactivation of Elongation Factor-1 Alpha in Sf21 Cell Lysates. Fourth International Symposium on Molecular Insect Science, May 28-June 2, 2002, Tucson, AZ, poster presentation. Abstract published in: ISMIS 2002. Abstracts of the Fourth International Symposium on Molecular Insect Science. 70pp. Journal of Insect Science, 2:17, Available online: insectscience.org/2.17.

2001 | Stuart MK, Chamberlain NR. Monoclonal Antibodies to Elongation Factor-1 Alpha Inhibit In Vitro Protein Translation in Lysates of Sf21 Cells; The Entomological Society of America 2001 Annual Meeting; San Diego, CA; December 10, 2001.

2001 | Stuart MK, Chamberlain NR: Production of Monoclonal Antibodies to Elongation Factor-1alpha from Spodoptera frugiperda; Rocky Mountain Conference of Entomologists; Woodland Park, CO; Aug. 5-9, 2001

2000 | Chamberlain NR. Identification and Partial Characterization of Factors That Inhibit Adherence of Staphylococcus epidermidis to Polystyrene; General Meeting of the American Society for Microbiology; Los Angeles, CA; May 21, 2000.

1999 | Smart JI, Chamberlain NR. 1999. Activator Protein and Cross-Strain Communication in Staphylococcus epidermidis. American Society for Microbiology-St. Louis .

1998 | Chamberlain NR. Bacterial Communications; Truman State University; Sept. 21, 1998.

1997 | Chamberlain NR. 1997. Characterization of FAME Expression in Staphylococcus epidermidis. 97th General meeting for the American Society for Microbiology .

2018 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW, EPA6-What Students Say When Physicians Ask Them to Call a Patient and Discuss Test Results, The AACOM Annual Conference, Washington DC. April 18-21, 2018

2018 | Chamberlain NR, Sexton PS, Hardee MR, Baer RW, Use of Physician Mentored Patient Rounds to Observe Second Year Medical Students’ Treatment Plans for a COPD Patient, Tenth Annual Interdisciplinary Biomedical Research Symposium, A.T. Still University (Kirksville Campus), MO. October 27, 2018

2018 | Curry EC and Chamberlain NR. Initial Characterization of Persistence Inducing Factor in Staphylococcus aureus SH 1000. American Society for Microbiology Microbe 2018, Atlanta, Georgia. June 7-10, 2018