Melissa Stuart, PhD

Interim Chair

Work: 660.626.2474
Fax: 660.626.2523
Email Address: mstuart@atsu.edu

Research Interests: Dr. Stuart investigates the immune response to protozoans of medical importance. Her current interests include evaluation of Trichomonas tenax as an oral pathogen, and the role of glycogen phosphorylase isoenzymes in the metabolism of Trichomonas vaginalis.

Education:

PhD - (Microbiology) University of Missouri-Columbia (1989)
MS - (Microbiology) Kansas State University
BS - (Biology) Missouri Western State College (1983)

Scholarly Activity

Research Publications:

2016 | Govro EJ [student], Stuart MK. Cytokine response of human THP-1 macrophages to Trichomonas tenax. Exp Parasitol. 2016 Aug 5;169:77-80. doi: 10.1016/j.exppara.2016.07.011. [Epub ahead of print]

2014 | Degenhardt BF, Johnson JC, Fossum C, Andicochea CT [student], Stuart MK. Changes in cytokines, sensory tests, and self-reported pain levels after manual treatment of low back pain. J Spinal Disord Tech. 2014 Dec 2. [Epub ahead of print]

2013 | Whitman JK, Alviar AF, Fleschner CR, Stuart MK. Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens. SpringerPlus 2013 Oct 2;2:500;13pp. doi:10.1186/2193-1801-2-500.

2012 | Chamberlain NR, Stuart MK, Singh VK, Sargentini NJ. Utilization of case presentations in medical microbiology to enhance relevance of basic science for medical students. Med Educ Online. 2012;17. doi: 10.3402/meo.v17i0.15943. Epub 2012 Mar 13

2012 | Stuart MK, Richardson NC, Singh VK, Sargentini NJ. Monoclonal Antibodies Against RadA/Sms Protein. Hybridoma (Larchmt). 2012 Feb;31(1):75

2012 | Richardson NC, Sargentini NJ, Singh VK, Stuart MK. Monoclonal antibodies against the Escherichia coli DNA repair protein RadA/Sms. Hybridoma (Larchmt). 2012 Feb;31(1):25-31

2008 | Van Meter KE, Stuart MK. A monoclonal antibody that inhibits translation in Sf21 cell lysates is specific for glyceraldehyde-3-phosphate dehydrogenase. Arch Insect Biochem Physiol. 2008 Oct 10;69(3):107-117

2008 | Singh VK, Hattangady DS, Giotis ES, Singh AK, Chamberlain NR, Stuart MK, Wilkinson BJ. Insertional inactivation of branched-chain {alpha}-keto acid dehydrogenase (BKD) in Staphylococcus aureus leads to decreased branched-chain membrane fatty acids content and increased susceptibility to certain stresses. Appl Environ Microbiol. 2008 Oct;74(19):5882-5890. Epub 2008 Aug. 8

2007 | Koch AJ, Wherry AD, Petersen MC, Johnson JC, Stuart MK, Sexton WL. Salivary immunoglobulin A response to a collegiate rugby game. J Strength Cond Res. 2007 Feb.;21(1):86-90

2006 | Habibi J, Goodman CL, Stuart MK. Distribution of elongation factor-1a in larval tissues of the fall armyworm, Spodoptera frugiperda. J. Insect Sci. 2006 October; 6:33, 9pp. Available online:

2005 | Stuart MK, Sexton PS, Cox BJ, Uray NJ, Towns LC. Monoclonal antibodies to the optic tectum and cerebellum of the bullfrog, Rana catesbeiana. Trans Missouri Acad Sci. 2005, 39:12-22

2004 | Noll DR, Degenhardt BF, Stuart M, McGovern R, Matteson M. Effectiveness of a sham protocol and adverse effects in a clinical trial of osteopathic manipulative treatment in nursing home patients. J Am Osteopath Assoc. 2004 Mar;104(3):107-13

2004 | Noll DR, Degenhardt BF, Stuart MK, Werden S, McGovern RJ, Johnson JC. The effect of osteopathic manipulative treatment on immune response to the influenza vaccine in nursing home residents: a pilot study. Altern Ther Health Med. 2004 Jul-Aug;10(4):74-6.

2003 | Hook RR Jr, Green TJ, Stuart MK. Rheumatoid factor-like IgM in Plasmodium berghei (Apicomplexa: Haemosporida) infections of BALB/c mice. Folia Parasitol (Praha). 2003 Sep;50(3):176-82

2003 | Stuart MK, Chamberlain NR. Monoclonal antibodies to elongation factor-1alpha inhibit in vitro translation in lysates of Sf21 cells. Arch Insect Biochem Physiol. 2003 Jan;52(1):17-34

1998 | Stuart MK. An antibody diagnostic for hymenopteran parasitism is specific for a homologue of elongation factor-1 alpha. Arch Insect Biochem Physiol 1998;39(1):1-8

1997 | Stuart MK, Greenstone, MH. 1997. Immunological detection of Hymenopteran parasitism in Helicoverpa zea and Heliothis virescens. Biol Control 8:197-202

1997 | Goodman CL, Greenstone MH, Stuart MK. 1997. Monoclonal antibodies to vitellins of Bollworm and Tobacco Budworm (Lepidoptera: Noctuidae): biochemical and ecological implications. Ann Entomol Soc Amer 90:83-90

1994 | Stuart MK, Barak AV, Burkholder WE. Immunological identification of Trogoderma granarium everts (Coleoptera: Dermestidae). J Stored Prod Res 1994. 30:9-16.

1991 | Stuart MK, Burkholder WE. Monoclonal antibodies specific for Laelius pedatus (Bethylidae) and Bracon hebetor (Braconidae), two hymenopterous parasitoids of stored-product pests. Biol. Control 1991. 1:302-8.

1991 | Greenstone MH, Stuart MK, Haunerland NH. Using monoclonal antibodies for phylogenetic analysis: an example from the Heliothinae (Lepidoptera: Noctuidae). Ann Entomol Soc Amer 1991. 84:457-64.

1990 | Stuart MK, Greenstone MH. Beyond ELISA: a rapid, sensitive, specific immunodot assay for identification of predator stomach contents. Ann Entomol Soc Amer 1990. 83:1101-7.

1990 | Stuart MK, Green TJ. Monoclonal IgM rheumatoid factor-like anti-globulins enhance the inhibitory effects of Plasmodium falciparum-specific monoclonal antibodies in vitro. Parasitology 1990 Oct;101 Pt 2:177-85

1989 | Stuart MK (PhD Thesis). The protective role of rheumatoid factor in immunity to malaria. University of Missouri-Columbia. 1989

1985 | Stuart MK (Masters Thesis). A comparative study of serum antibody specificities and antigenic differences among strains as contributing factors to chronic infection with Giardia lamblia in humans. Kansas State University. 1985

Books, Chapters, and Reviews:

1996 | Stuart MK, Greenstone MH. 1996. Serological diagnosis of parasitism: a monoclonal antibody-based immunodot assay for Microplitis croceipes (Hymenoptera: Braconidae), pp. 301-321. In WOC Symondson, E Liddell (eds.), The Ecology of Agricultural Pests - Biochemical Approaches, Chapman and Hall

Presentations and Published Abstracts:

2016 | Govro EJ [student], Stuart MK. Cytokine Response of Human THP-1 Macrophages to Trichomonas tenax (Trichomonadida: Trichomonadidae). Poster presented at the Annual Midwestern Conference of Parasitologists (AMCOP 68), Southern Illinois University, Carbondale, IL, June 9-11, 2016.

2016 | Nachtrab SN, Hudman DA, Hiatt JL, Sargentini NJ, Stuart MK. Fine Epitope Mapping of Monoclonal Antibodies to the RadA DNA Repair Protein. Poster presented at the 8th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 5, 2016.

2016 | Sargentini NJ, Chamberlain NR, Hudman DA (staff)), Phillips PL, Singh VK, Stuart MK. Assessing Medical Student Critical Thinking and Interpersonal Skills along with Medical Microbiology, Immunology and Infectious Diseases Knowledge. Poster presented at Assessment Week; A.T. Still University of Health Sciences; Kirksville, MO; February 9, 2016.

2016 | Stuart MK [invited speaker], Chamberlain NR, Singh VK, Hudman DA [staff], Phillips PL, Sargentini NJ. The Small-Group Clinical Case Presentation: An Instrument for Assessing Interpersonal Skills, Critical Thinking, and Knowledge in Medical Microbiology, Immunology, and Infectious Diseases. Oral presentation at the 16th Educational Strategies Workshop; Palm Springs, CA; May 15-18, 2016.

2015 | Govro EJ (Student), Stuart MK. Cytokines Synthesized by THP-1 Macrophages in Response to Trichomonas tenax. Poster presented at American Society for Microbiology, 115th General Meeting; New Orleans, LA; May 30-June 2, 2015.

2015 | Govro EJ [student], Melissa K. Stuart. Cytokine Production by THP-1 Macrophages Stimulated with Trichomonas tenax. Poster presented at the 7th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 31, 2015.

2015 | Lighthouse NC [student], Brittingham A, Wilson WA, Stuart MK. Anti-Peptide Sera Differentiate Between Glycogen Phosphorylase Isoenzymes of Trichomonas vaginalis. Poster presented at American Society for Microbiology, 115th General Meeting; New Orleans, LA; May 30-June 2, 2015.

2015 | Sargentini NJ, Chamberlain NR, Hudman DA (Staff), Phillips PL, Singh VK, Stuart MK. Assessing Critical Thinking and Interpersonal Skills along with Medical Microbiology, Immunology and Infectious Diseases Knowledge. Poster presented at Assessment Week; A. T. Still University of Health Sciences; April 13-16, 2015.

2015 | Whitman JK [resident], Alviar AF, Fleschner CR, Stuart MK. Monoclonal Antibody 10A5 Recognizes an Antigen Unique to the Water-Insoluble 25/45 Fraction of the Rat Ocular Lens. Poster presented at 99th Annual Clinical Assembly of the American Osteopathic Colleges of Ophthalmology and Otolaryngology-Head and Neck Surgery Foundation, Inc., Orlando, FL; May 6-10, 2015.

2014 | Govro EJ [student], Stuart MK. Growth Characteristics of THP-1 and Trichomonas tenax Cells In Vitro. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2014 | Lighthouse NC [student], Brittingham A, Wilson WA, Stuart MK. Antibodies to Trichomonas vaginalis Glycogen Phosphorylase Isoenzymes. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2014 | Stuart MK, Hudman DA [staff], Hiatt JL [student], Sargentini NJ. Recognition of radA Ochre Mutants by Monoclonal Antibody 2A2. Poster presented at American Society for Microbiology, 114th General Meeting; Boston, MA; May 17-20, 2014.

2014 | Whitman JK [resident], Fleschner CR, Stuart MK. Isolation of a Ganglioside Unique to the Water-Insoluble Membrane Fraction of Rat Lens. Poster presented at the 3rd Annual Missouri Osteopathic Student and Post-graduate Research Symposium; Missouri Osteopathic Annual Convention; Branson, MO; April 30-May 4, 2014.

2013 | Chamberlain NR, Stuart MK, Singh VK, Sargentini NJ. Utilization of Clinical Case Presentations in Medical Microbiology and Infectious Diseases to Improve Student Performance on Course Assessments. Poster presented at Annual Meeting of the American Association of Colleges of Osteopathic Medicine; Baltimore, MD; April 24, 2013.

2013 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ. Case Presentation Exercises in Immunology Enhance Relevance and Improve Student Satisfaction with the Course. Poster presented at American Society for Microbiology, 113th General Meeting; Denver, CO; May 18-21, 2013.

2013 | Stuart MK, Hudman DA [staff], Pisano MD, Hiatt JL [student], Sargentini NJ. Recognition of radA ochre Mutants by RadA/Sms-Specific Monoclonal Antibodies. Poster presented at 5th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 2, 2013.

2012 | Hiatt JL [student], Sargentini NJ, Stuart MK. The Effect of Alanine Substitutions on RadA/Sms Epitope Stability. Poster presented at the 112th General Meeting of the American Society for Microbiology; June 16-19, 2012; San Francisco, California.

2012 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ. Implementation of a Small Group Clinical Case Presentation Exercise in Immunology. AMSMIC 14th Educational Strategies Workshop; Santa Fe, NM. April 28-May 2, 2012.

2012 | Stuart MK, Hiatt JL [student], Sargentini NJ. Epitope Mapping of RadA/Sms-Specific Monoclonal Antibodies by Competitive ELISA. Poster presented at 4th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; September 29, 2012.

2011 | Stuart MK, Richardson NC, Hiatt JL [student], Singh VK, Sargentini NJ. Analysis of Proteins that Co-precipitate with RadA/Sms in X-irradiated Escherichia coli. Poster presented at the 111th General Meeting of the American Society for Microbiology; New Orleans, LA; May 21-24, 2011.

2011 | Stuart MK, Hiatt JL [student], Richardson NC [student], Sargentini NJ. The Effect of Alanine Substitutions on RadA/Sms Epitope Stability. Poster presented at 3rd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 22, 2011.

2010 | Richardson NC [student], Sargentini NJ, Singh VK, Stuart MK; Production of Monoclonal Antibodies to the Escherichia coli DNA Repair Protein, RadA/Sms (poster presentation). 110th General Meeting of the American Society for Microbiology; May 23-27, 2010; San Diego, CA.

2010 | Stuart MK, Richardson NC [student], Hiatt JL [student], Singh VK, Sargentini NJ. Analysis of Proteins that Co-precipitate with RadA/Sms in X-irradiated Escherichia coli. Poster presented at 2nd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 9, 2010.

2010 | Whitman JK [student], Fleschner CR, Stuart MK; Isolation of a Ganglioside Unique to the Water-Insoluble Fraction of Ocular Lens; Association for Research in Vision and Ophthalmology Annual Meeting; Fort Lauderdale, FL; May 2-6, 2010

2009 | Stuart MK, Chamberlain NR, Singh VK, Sargentini NJ; Microbiology for Medical Students: A Case Presentations Model; 109th General Meeting of the American Society for Microbiology; Philadelphia, PA; May 17-21, 2009.

2008 | Degenhardt BF, Stuart MK, Yang Q, Johnson JC, Fossum C; Serum Proinflammatory Markers Unable to Differentiate Between Subjects With and Without Low Back Pain; 12th World Congress on Pain; Glasgow, Scotland, UK; Aug. 17 - 22, 2008.

2008 | Singh VK, Hattangady D, Giotis E, Chamberlain NR, Stuart MK, Wilkinson B; Insertional Inactivation of Branched-chain Alpha-keto Acid Dehydrogenase (BKD) in Staphylococcus aureus Leads to Decreased Branched-chain Membrane Fatty Acids Content and Increased Susceptibility to Certain Stresses; International Symposium on Staphylococci and Staphylococcal Infections; Cairns, Australia; Sept. 7 - 10, 2008.

2007 | Alviar AF, Fleschner CR, Stuart MK; Fragmented Forms of Galectin Related Inter-fiber Protein are Uniquely Associated with the Water Insoluble Membrane Fraction of Normal Rat Lens; 47th Annual Meeting of the American Society for Cell Biology; Washington, D.C.; Dec. 1-5, 2007. Sargentini NJ, McNabb JE, Higbee D, Stuart MK, Chamberlain NR, Lockwood MD; Using Standardized Patient Encounters To Improve Basic Science Understanding And Integrate Professional Behavior And Clinical Skills Assessment Within Two Medical School Basic Science Courses; Central Group on Educational Affairs, Association of American Medical Colleges Spring Conference; Indianapolis, IN; March 22-24, 2007, poster presentation. Stuart MK; Does Glyceraldehyde-3-Phosphate Dehydrogenase From Spodoptera Frugiperda Share An Epitope With Elongation Factor-1α?; Joint Meeting of the North Central Branch of the Entomological Society of America and Entomological Society of Manitoba; Winnipeg, MB, Canada; March 25-28, 2007.

2006 | Habibi J, Goodman CL, Stuart MK; Distribution of Elongation Factor-1a in Larval Tissues of the Fall Armyworm, Spodoptera frugiperda; Fifth International Symposium of Insect Molecular Science; Tucson, AZ; May 20-24, 2006.

2005 | Habibi J, Goodman CL, Stuart MK. Distribution of Elongation Factor-1alpha in Larval Tissues of the Fall Armyworm, Spodoptera frugiperda. Missouri Life Sciences Week 2005, Columbia, MO; April 11-15, 2005.

2004 | Van Meter KE, Stuart MK. Evaluation of Commercial Protein Transduction Reagents for Use in an Insect Cell Line. 2004 Entomological Society of America Annual Meeting, Salt Lake City, UT; Nov 14-17, 2004.

2003 | Stuart MK, Sexton PS, Cox BJ, Uray NJ, Towns LC. Monoclonal Antibodies to the Bullfrog Optic Tectum and Cerebellum. Missouri Academy of Sciences Annual Meeting; Warrensburg, MO; April 19, 2003.

2003 | Stuart MK. A role for Baculovirus-Encoded Antibodies in the Biological Control of Pest Insects. Invited seminar; Biology Department, Northern Michigan University; Marquette, MI; May 1, 2003.

2003 | Wherry AD, Petersen MC, Sexton WL, Stuart MK, Koch AJ. Decreased Salivary Immunoglobulin Levels After a Collegiate Rugby Game. Med Sci Sports Exercise 35 (Suppl): S381, 2003. American College of Sports Medicine meetings; San Francisco, CA; May 31, 2003.

2002 | Murphy TL, Stuart, M.K. Characterization of Monoclonal Antibodies to Elongation Factor-1a by Peptide Mapping. Annual Meeting of the Entomological Society of America; November 14-20, 2002; Ft. Lauderdale, FL

2002 | Stuart MK, Chamberlain NR. 2002. Immunological Inactivation of Elongation Factor-1 Alpha in Sf21 Cell Lysates. Fourth International Symposium on Molecular Insect Science, May 28-June 2, 2002, Tucson, AZ, poster presentation. Abstract published in: ISMIS 2002. Abstracts of the Fourth International Symposium on Molecular Insect Science. 70pp. Journal of Insect Science, 2:17, Available online

2001 | Stuart MK, Chamberlain NR. Monoclonal Antibodies to Elongation Factor-1 Alpha Inhibit In Vitro Protein Translation in Lysates of Sf21 Cells; The Entomological Society of America 2001 Annual Meeting; San Diego, CA; December 10, 2001.

2001 | Stuart MK, Chamberlain NR: Production of Monoclonal Antibodies to Elongation Factor-1alpha from Spodoptera frugiperda; Rocky Mountain Conference of Entomologists; Woodland Park, CO; Aug. 5-9, 2001

2001 | Stuart MK. Production of Monoclonal Antibodies to Elongation Factor-1a from Spodoptera frugiperda; 72nd Annual Meeting of the Rocky Mountain Conference of Entomologists; Woodland Park, CO; August 6, 2001.

2001 | Werden S, Noll DR, Stuart MK, Johnson JC, Degenhardt BF. 2001. Quantitative Analysis of Elderly Immune Responses to Influenza Vaccination with Osteopathic Manipulation. Alternative Therapies in Health and Medicine Vol. 7, No. 3.

2000 | Stuart MK. Partial Purification of Elongation Factor-1 Alpha From Sf21 Cells; JAM - The Joint Annual Meeting of the Entomological Society of Canada, Societe d'Entomologie du Quebec, and the Entomological Society of America; Montreal, Quebec, Canada; Dec. 3, 2000.

2000 | Werden S, Noll DR, Stuart MK, Johnson JC, Degenhardt BF. 2000. Qualitative Analysis of Elderly Immune Responses to Influenza Vaccination with Osteopathic Manipulation. JAOA 100:513.

1999 | Stuart MK. 1999. Production of Monoclonal Antibodies to Noctuid Elongation Factor-1. Entomological Society of America.

1998 | Stuart MK. 1998. An Antibody Diagnostic for Hymenopteran Endoparasitism is Specific for Elongation Factor-1Alpha. Program Guide & Abstracts for the American Society of Parasitologists (Kona, Hawaii).

1997 | Stuart MK. 1997. Monoclonal Antibody-based Detection of Interspecific Insect Parasitism. 49th Annual Midwestern Conference of Parasitologists(Indianapolis, IN).