Patricia B. Lodato, PhD
Assistant ProfessorResearch Interests: Dr. Lodato is interested in understanding how the expression of virulence factors is regulated in the bacterial pathogen enterohemorrhagic Escherichia coli (EHEC). EHEC can produce diarrhea, bloody diarrhea, or more serious conditions that result in kidney failure, neurological complications and even death. The critical virulence trait of EHEC is the production of a potent toxin, called Shiga toxin, for which there is no antidote. Since treatment with conventional antibiotics is currently contraindicated in the USA, only supportive therapy, such as administration of fluids, is available for patients infected with EHEC. The production of toxin is triggered when a prophage, which is a bacterial virus inserted into the EHEC genome, excises from the chromosome and initiates replication and the production of phage particles. Currently, Dr. Lodato’s laboratory is determining what bacterial factors, such as ribonucleases, influence the production of toxin and affect phage induction. Another line of research concerns the role of ribonucleases on EHEC survival and adaptation under the environmental conditions prevailing in the intestine, for example, anaerobiosis.
Education:
BS - Biological Sciences, University of Buenos Aires (1991)PhD - Microbiology, University of Chile (2003)
Postdoctoral Fellow - University of Maryland Baltimore County (2003-2005)
Postdoctoral Fellow - University of Maryland School of Medicine (2005-2009)
Research Associate-Faculty rank - University of Maryland School of Medicine (2009-2013)
Assistant Professor - New Mexico State University (2013-2019)
Assistant Professor - A.T. Still University (2019-present)
Scholarly Activity
Research Publications:
2021 |
Lodato, P.B. The effect of two ribonucleases on the production of Shiga toxin and stx-bearing bacteriophages in enterohaemorrhagic Escherichia coli. Under revision.
2018 | Thuraisamy T, Lodato PB. Influence of RNase E deficiency on the production of stx2-bearing phages and Shiga toxin in an RNase E-inducible strain of enterohaemorrhagic Escherichia coli (EHEC) O157:H7. J Med Microbiol. 2018. 67(5):724-732. doi: 10.1099/jmm.0.000728. Epub 2018 Apr 5. PMID: 29620505; PMCID: PMC7001489.
2017 | Lodato PB, Thuraisamy T, Richards J, Belasco JG. Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7. FEMS Microbiol Lett. 2017, 364(13):fnx131. doi: 10.1093/femsle/fnx131. PMID: 28854682; PMCID: PMC5827626.
2012 | Lodato PB, Hsieh PK, Belasco JG, Kaper JB. The ribosome binding site of a mini-ORF protects a T3SS mRNA from degradation by RNase E. Mol Microbiol. 2012, 86(5):1167-82. doi: 10.1111/mmi.12050. Epub 2012 Oct 12. PMID: 23043360; PMCID: PMC3537511.
2012 | Lodato PB, Kaper JB. Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli [published correction appears in Mol Microbiol. 2012 Jun;84(5):990]. Mol Microbiol. 2009;71(2):273-290. doi:10.1111/j.1365-2958.2008.06530.x
2009 | Baeza M, Retamales P, Sepúlveda D, Lodato P, Jiménez A, Cifuentes V. Isolation, characterization and long-term preservation of mutant strains of Xanthophyllomyces dendrorhous. J Basic Microbiol. 2009, 49(2):135-41. doi: 10.1002/jobm.200800096. PMID: 18792046.
2008 | Niklitschek M, Alcaíno J, Barahona S, Sepúlveda D, Lozano C, Carmona M, Marcoleta A, Martínez C, Lodato P, Baeza M, Cifuentes V. Genomic organization of the structural genes controlling the astaxanthin biosynthesis pathway of Xanthophyllomyces dendrorhous. Biol Res. 2008, 41(1):93-108. doi: 10.4067/S0716-97602008000100011. Epub 2008 Aug 21. PMID: 18769767.
2007 | Lodato P, Alcaíno J, Barahona S, Niklitschek M, Carmona M, Wozniak A, Baeza M, Jiménez A, Cifuentes V. Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous. Biol Res. 2007;40(1):73-84. doi: 10.4067/s0716-97602007000100008. Epub 2007 Jul 19. PMID: 17657357.
2006 | Lodato PB, Rogers EJ, Lovett PS. A variation of the translation attenuation model can explain the inducible regulation of the pBC16 tetracycline resistance gene in Bacillus subtilis. J Bacteriol. 2006, 188(13):4749-58. doi: 10.1128/JB.01937-05. PMID: 16788184; PMCID: PMC1482984.
2004 | Lodato P, Alcaíno J, Barahona S, Retamales P, Jiménez A, Cifuentes V. Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma). Biol Res. 2004,37(1):83-93. doi: 10.4067/s0716-97602004000100009. PMID: 15174308.
2004 | Cotorás D, Tantaleán JC, and Lodato P. Aplicación de un acercamiento molecular para la identificación in situ de bacterias en una biopelícula marina. (Molecular approach for in situ identification of bacteria in a marine biofilm). 2004. In Metal 2001 (ed., MacLeod ID, Theile JM, and Degrigny C), Western Australian Museum, ISBN 1920843175.
2003 | Lodato P, Alcaino J, Barahona S, Retamales P, Cifuentes V. Alternative splicing of transcripts from crtI and crtYB genes of Xanthophyllomyces dendrorhous. Appl Environ Microbiol. 2003, 69(8):4676-82. doi: 10.1128/AEM.69.8.4676-4682.2003. PMID: 12902257; PMCID: PMC169127.
1999 | Mazzobre MF, Lodato P, Segovia de Huergo M., Buera MP, and Chirife J. The performance of maltodextrins as protective excipients in freeze-dried biomaterials subjected to thermal treatment, in comparison with trehalose systems. 1999. In Biopolymer Science: Food and non-food applications (ed. Colonna, P & Guilbert S), INRA Editions, Montpellier, France. ISBN: 2-7380-0874-7.
1999 | Lodato P, Segovia de Huergo M, Buera MP. Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices. Appl Microbiol Biotechnol. 1999, 52(2):215-20. doi: 10.1007/s002530051511. PMID: 10499261.
1997 | Lodato PB, Forchiassin F, Segovia de Huergo MB. Producción de amilasas por Aureobasidium pullulans en medio líquido y sólido [Amylase production by Aureobasidium pullulans in liquid and solid media]. Rev Argent Microbiol. 1997 Jul-Sep;29(3):115-21. Spanish. PMID: 9411486.
2018 | Thuraisamy T, Lodato PB. Influence of RNase E deficiency on the production of stx2-bearing phages and Shiga toxin in an RNase E-inducible strain of enterohaemorrhagic Escherichia coli (EHEC) O157:H7. J Med Microbiol. 2018. 67(5):724-732. doi: 10.1099/jmm.0.000728. Epub 2018 Apr 5. PMID: 29620505; PMCID: PMC7001489.
2017 | Lodato PB, Thuraisamy T, Richards J, Belasco JG. Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7. FEMS Microbiol Lett. 2017, 364(13):fnx131. doi: 10.1093/femsle/fnx131. PMID: 28854682; PMCID: PMC5827626.
2012 | Lodato PB, Hsieh PK, Belasco JG, Kaper JB. The ribosome binding site of a mini-ORF protects a T3SS mRNA from degradation by RNase E. Mol Microbiol. 2012, 86(5):1167-82. doi: 10.1111/mmi.12050. Epub 2012 Oct 12. PMID: 23043360; PMCID: PMC3537511.
2012 | Lodato PB, Kaper JB. Post-transcriptional processing of the LEE4 operon in enterohaemorrhagic Escherichia coli [published correction appears in Mol Microbiol. 2012 Jun;84(5):990]. Mol Microbiol. 2009;71(2):273-290. doi:10.1111/j.1365-2958.2008.06530.x
2009 | Baeza M, Retamales P, Sepúlveda D, Lodato P, Jiménez A, Cifuentes V. Isolation, characterization and long-term preservation of mutant strains of Xanthophyllomyces dendrorhous. J Basic Microbiol. 2009, 49(2):135-41. doi: 10.1002/jobm.200800096. PMID: 18792046.
2008 | Niklitschek M, Alcaíno J, Barahona S, Sepúlveda D, Lozano C, Carmona M, Marcoleta A, Martínez C, Lodato P, Baeza M, Cifuentes V. Genomic organization of the structural genes controlling the astaxanthin biosynthesis pathway of Xanthophyllomyces dendrorhous. Biol Res. 2008, 41(1):93-108. doi: 10.4067/S0716-97602008000100011. Epub 2008 Aug 21. PMID: 18769767.
2007 | Lodato P, Alcaíno J, Barahona S, Niklitschek M, Carmona M, Wozniak A, Baeza M, Jiménez A, Cifuentes V. Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous. Biol Res. 2007;40(1):73-84. doi: 10.4067/s0716-97602007000100008. Epub 2007 Jul 19. PMID: 17657357.
2006 | Lodato PB, Rogers EJ, Lovett PS. A variation of the translation attenuation model can explain the inducible regulation of the pBC16 tetracycline resistance gene in Bacillus subtilis. J Bacteriol. 2006, 188(13):4749-58. doi: 10.1128/JB.01937-05. PMID: 16788184; PMCID: PMC1482984.
2004 | Lodato P, Alcaíno J, Barahona S, Retamales P, Jiménez A, Cifuentes V. Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma). Biol Res. 2004,37(1):83-93. doi: 10.4067/s0716-97602004000100009. PMID: 15174308.
2004 | Cotorás D, Tantaleán JC, and Lodato P. Aplicación de un acercamiento molecular para la identificación in situ de bacterias en una biopelícula marina. (Molecular approach for in situ identification of bacteria in a marine biofilm). 2004. In Metal 2001 (ed., MacLeod ID, Theile JM, and Degrigny C), Western Australian Museum, ISBN 1920843175.
2003 | Lodato P, Alcaino J, Barahona S, Retamales P, Cifuentes V. Alternative splicing of transcripts from crtI and crtYB genes of Xanthophyllomyces dendrorhous. Appl Environ Microbiol. 2003, 69(8):4676-82. doi: 10.1128/AEM.69.8.4676-4682.2003. PMID: 12902257; PMCID: PMC169127.
1999 | Mazzobre MF, Lodato P, Segovia de Huergo M., Buera MP, and Chirife J. The performance of maltodextrins as protective excipients in freeze-dried biomaterials subjected to thermal treatment, in comparison with trehalose systems. 1999. In Biopolymer Science: Food and non-food applications (ed. Colonna, P & Guilbert S), INRA Editions, Montpellier, France. ISBN: 2-7380-0874-7.
1999 | Lodato P, Segovia de Huergo M, Buera MP. Viability and thermal stability of a strain of Saccharomyces cerevisiae freeze-dried in different sugar and polymer matrices. Appl Microbiol Biotechnol. 1999, 52(2):215-20. doi: 10.1007/s002530051511. PMID: 10499261.
1997 | Lodato PB, Forchiassin F, Segovia de Huergo MB. Producción de amilasas por Aureobasidium pullulans en medio líquido y sólido [Amylase production by Aureobasidium pullulans in liquid and solid media]. Rev Argent Microbiol. 1997 Jul-Sep;29(3):115-21. Spanish. PMID: 9411486.
Presentations and Published Abstracts:
2021 |
Lodato PB. Deficiency of ribonuclease (RNase) E and lack of RNase G impair the production of Shiga toxin and phages in enterohemorrhagic Escherichia coli (EHEC). The Microbial Pathogenesis Conference: Mechanisms of Infectious Disease, Federation of American Societies for Experimental Biology, July 13-15, 2021. Virtual. Poster presentation.
2020 | Lodato PB. Transcriptome analysis of pathogenic E. coli, 12th Annual Virtual Interdisciplinary Biomedical Research Symposium, ATSU, Kirksville, MO, 11/07/2020. Oral presentation.
2019 | Lodato PB. Influence of ribonucleases E and G on the production of phage and Shiga toxin in enterohemorrhagic Escherichia coli (EHEC) O157:H7. 11th Annual Interdisciplinary Biomedical Research Symposium, ATSU, Kirksville, MO, 11/2/2019. Poster presentation.
2018 | Lodato PB. Thuraisamy T [student]. Influence of ribonucleases E and G on the production of phage and Shiga toxin in enterohemorrhagic Escherichia coli (EHEC) O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 08/02/2018. Oral presentation.
2018 | Thuraisamy T [student], Lodato PB. Deficiency of ribonuclease E (RNase E) impairs the production of infectious Shiga toxin phage in enterohemorrhagic E. coli (EHEC). 17th Annual URC Research & Creative Activities Fair, 04/04/2018. Poster presentation.
2018 | Shoots A [student], Lodato PB. Effect of ribonuclease E (RNase E) deficiency in a mediator of the SOS response in enterohemorrhagic Escherichia coli (EHEC) O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 08/02/2018. Poster presentation.
2018 | Ortega F [student], Sundararajan A, Ramaraj T, Schilkey F, Lodato PB. Transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157:H7 containing low concentrations of ribonuclease E (RNase E). Annual Symposium NM-INBRE, Santa Fe, NM, 08/01/2018. Poster presentation.
2018 | Thuraisamy T [student], Lodato PB. Effect of RNase E and RNase G on Shiga toxin production in enterohemorrhagic Escherichia coli (EHEC) O157:H7. ASM Tri-Branch Meeting, Durango, CO, 04/6-7/2018. Poster presentation.
2017 | Das S [student], Lodato PB. Bile reduces growth of enterohemorragic Escherichia coli (EHEC) O157:H7 deficient in RNase E. ASM Rio Grande Branch Annual Meeting, Las Cruces, NM, 04/08/2017. Poster presentation.
2017 | Chan R [student], Das S [student], Lodato PB. Effect of bile and pH in enterohemorrhagic Escherichia coli (EHEC) deficient in RNase E. Annual Symposium NM-INBRE, Santa Fe, NM, 03/18/2017. Poster presentation.
2016 | Thuraisamy T [student], Richards J, Belasco JG, Lodato PB. Reduced ribonuclease E levels impair Shiga Toxin 2 phage production in enterohemorrhagic E. coli (EHEC) O157:H7. Molecular Genetics of Bacteria and Phages Meeting, University of Wisconsin, Madison, 08/08/2016. Poster presentation.
2016 | Thuraisamy T [student], Belasco JG, PB Lodato. Effects of RNase E on Shiga toxin phage production and type 3 secretion in E. coli O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 03/2016. Oral presentation.
2015 | Lodato PB, Richards J, and Belasco JG. Influence of RNase E on enterohemorrhagic E. coli O157:H7. The 9th Triennial International Symposium on Shiga Toxin Producing Escherichia coli Infections, Boston, MA, 09/13-16/2015. Poster presentation.
2020 | Lodato PB. Transcriptome analysis of pathogenic E. coli, 12th Annual Virtual Interdisciplinary Biomedical Research Symposium, ATSU, Kirksville, MO, 11/07/2020. Oral presentation.
2019 | Lodato PB. Influence of ribonucleases E and G on the production of phage and Shiga toxin in enterohemorrhagic Escherichia coli (EHEC) O157:H7. 11th Annual Interdisciplinary Biomedical Research Symposium, ATSU, Kirksville, MO, 11/2/2019. Poster presentation.
2018 | Lodato PB. Thuraisamy T [student]. Influence of ribonucleases E and G on the production of phage and Shiga toxin in enterohemorrhagic Escherichia coli (EHEC) O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 08/02/2018. Oral presentation.
2018 | Thuraisamy T [student], Lodato PB. Deficiency of ribonuclease E (RNase E) impairs the production of infectious Shiga toxin phage in enterohemorrhagic E. coli (EHEC). 17th Annual URC Research & Creative Activities Fair, 04/04/2018. Poster presentation.
2018 | Shoots A [student], Lodato PB. Effect of ribonuclease E (RNase E) deficiency in a mediator of the SOS response in enterohemorrhagic Escherichia coli (EHEC) O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 08/02/2018. Poster presentation.
2018 | Ortega F [student], Sundararajan A, Ramaraj T, Schilkey F, Lodato PB. Transcriptome analysis of enterohemorrhagic Escherichia coli (EHEC) O157:H7 containing low concentrations of ribonuclease E (RNase E). Annual Symposium NM-INBRE, Santa Fe, NM, 08/01/2018. Poster presentation.
2018 | Thuraisamy T [student], Lodato PB. Effect of RNase E and RNase G on Shiga toxin production in enterohemorrhagic Escherichia coli (EHEC) O157:H7. ASM Tri-Branch Meeting, Durango, CO, 04/6-7/2018. Poster presentation.
2017 | Das S [student], Lodato PB. Bile reduces growth of enterohemorragic Escherichia coli (EHEC) O157:H7 deficient in RNase E. ASM Rio Grande Branch Annual Meeting, Las Cruces, NM, 04/08/2017. Poster presentation.
2017 | Chan R [student], Das S [student], Lodato PB. Effect of bile and pH in enterohemorrhagic Escherichia coli (EHEC) deficient in RNase E. Annual Symposium NM-INBRE, Santa Fe, NM, 03/18/2017. Poster presentation.
2016 | Thuraisamy T [student], Richards J, Belasco JG, Lodato PB. Reduced ribonuclease E levels impair Shiga Toxin 2 phage production in enterohemorrhagic E. coli (EHEC) O157:H7. Molecular Genetics of Bacteria and Phages Meeting, University of Wisconsin, Madison, 08/08/2016. Poster presentation.
2016 | Thuraisamy T [student], Belasco JG, PB Lodato. Effects of RNase E on Shiga toxin phage production and type 3 secretion in E. coli O157:H7. Annual Symposium NM-INBRE, Santa Fe, NM, 03/2016. Oral presentation.
2015 | Lodato PB, Richards J, and Belasco JG. Influence of RNase E on enterohemorrhagic E. coli O157:H7. The 9th Triennial International Symposium on Shiga Toxin Producing Escherichia coli Infections, Boston, MA, 09/13-16/2015. Poster presentation.
