Michael W. Bradbury, PhD

Chair

Work: 660.626.2761
Fax: 660.626.2981

Research Interests: Dr. Bradbury’s research interests center on uptake of fatty acids into cells and the function of fatty acid transporters, especially in the setting of obesity and hepatic steatosis. Understanding these processes and molecules will help us describe the processes whereby excess fats accumulate in adipose and liver tissue, leading to obesity, diabetes and fatty liver disease of various types. This knowledge could lead to better treatments for disorders and interventions that may prevent their development.

Education:

PhD - Yale University (1982)
MPhil - Yale University (1979)
BS - Rochester Institute of Technology (1976)

Scholarly Activity

Research Publications:

| Carter, ML, Bradbury MW, 2016. Oxygen Isotope Ratios in Primate Bone Carbonate Reflect Amount of Leaves and Vertical Stratification in the Diet. Am. J. Primatol., 78:1086-1097 (doi:10.1002/ajp.22432)

| Bradbury MW, Stump D, Guarnieri F, Berk PD, 2011. Molecular modeling and confirmation of a predicted fatty acid binding site of mitochondrial aspartate aminotransferase. J. Mol. Biol., 412:412-422 (doi:10.1016/j.jmb.2011.07.034)

| Rogers RW, Tranovich MJ, Bradbury MW, Habusta SF, 2011. Foot Deformities and Pain in the Geriatric Patient, J Penn Osteopathic Med Assoc, 55, No. 4:15-20

| Petrescu O, Cheema AF, Fan X, Bradbury MW, Berk PD, 2008. Differences in Adipocyte Uptake of Long Chain Fatty Acids in Response to High Fat Diets in Osborne-Mendel and S5B/Pl Rats. Int J Obes 32: 853-862 (doi:10.1038/sj.ijo.0803792)

| Bradbury MW, 2006. Lipid Metabolism and Liver Inflammation. I. Hepatic Fatty Acid Uptake: Possible Role in Steatosis. Am. J. Physiol. Gastrointest. Liver Physiol. 290:G194-8.

| Berk, PD, Zhou, SL, Bradbury, MW, 2005. Increased hepatocellular uptake of long chain fatty acids occurs by different mechanisms in fatty livers due to obesity or excess ethanol use, contributing to development of steatohepatitis in both settings. Transact Amer Clin Climatolog Assoc 116:335-345.

| Petrescu O, Fan X, Gentileschi P, Hossain S, Bradbury M, Gagner M, Berk PD, 2005. Long chain fatty acid uptake is upregulated in omental adipocytes from patients undergoing bariatric surgery for obesity. Int. J. Obes. Relat. Metab.Disord. 29:196-203

| Bradbury MW, Berk PD, 2004. Lipid metabolism in hepatic steatosis. Clinics. Liver Dis. 8:639-671

| Bradbury, MW, Berk,PD, 2004. Cellular uptake of long chain free fatty acids: the structure and function of plasma membrane fatty acid binding protein. Adv.Mol.Cell Biol. 33:47-81.

| Fan X, Bradbury MW, Berk PD, 2003. Leptin and insulin modulate nutrient partitioning and weight loss in ob/ob mice through regulation of long-chain fatty acid uptake by adipocytes. J. Nutrition 133:2707-2715.

| Bradbury, MW and PD Berk, 2000. Mitochondrial aspartate aminotransferase: direction of a single protein with two distinct functions to two sub-cellular sites does not require alternative splicing of the mRNA. Biochem. J. 345:423-427

| Berk PD, Zhou S, Kiang C, Stump DD, Fan X, Bradbury MW, 1999. Selective up-regulation of fatty acid uptake by adipocytes characterizes both genetic and diet-induced obesity in rodents. J.Biol.Chem. 274:28626-28631.

| Zhou, S-L, RE Gordon, M Bradbury, D Stump, C-L Kiang and PD Berk, 1998. Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells. Hepatology 27:1067-1074.

| Berk, PD, S-L Zhou, M Bradbury, D Stump, C-L Kiang, and LM Isola, 1997. Regulated membrane transport of free fatty acids in adipocytes: Role in obesity and non-insulin dependent diabetes mellitus. Trans. Amer. Clin. Climat Assoc. 108:26-43.

| Berk, PD, S-L Zhou, C-L Kiang, D Stump, M Bradbury, and LM Isola, 1997. Uptake of long chain free fatty acids is selectively upregulated in adipocytes of Zucker rats with genetic obesity and non-insulin dependent diabetes mellitus. J. Biol. Chem. 272:8830-8835.

| Berk, PD, S-L Zhou, M Bradbury, D Stump, C-L Kiang, and N-I Han, 1996. Crigler-Najjar Syndrome and the jaundiced Gunn rat: Model diseases for the evaluation of novel molecular and cellular therapies. In: HE Blum, GY Yu and CH Wu, editors, Molecular Diagnosis and Gene Therapy. Kluwer, Lancaster (UK). pp 223-244.

| Berk, PD, M Bradbury, S-L Zhou, D Stump and N-I Han, 1996. Characterization of membrane transport processes: Lessons from the study of BSP, bilirubin and fatty acid uptake. Seminars in Liver Diseases 16:107-120.

| Isola, LM, S-L Zhou, C-L Kiang, DD Stump, MW Bradbury and PD Berk, 1995. 3T3 fibroblasts transfected with a cDNA for mitochondrial aspartate aminotransferase express plasma membrane fatty acid-binding protein and saturable fatty acid uptake. PNAS 92:9866-9870

| Fabricant JD, Chalmers JH Jr., Bradbury MW, 1995. Bioluminescent Strains of E. coli for the Assay of Biocides. Bull. Environ. Contam. Toxicol. 54:90-95

| Gordon, JW and MW Bradbury, 1991. Genomic imprinting - a phenomenon with implications for micromanipulation-assisted IVF. J. IVF & Emb. Trans. 8:5-14

| Gordon JW, Isola LM, Bradbury MW, 1990. Rapid Sexing of Preimplantation Mouse Embryos by Blastomere Biopsy and Enzymatic Amplification, Advances in Assisted Reproductive Technologies, pp 871-886

| Bradbury, MW, L Isola and J Gordon, 1990. Enzymatic amplification of a Y-chromosome repeat in a single blastomere allows identification of the sex of preimplantation mouse embryos. PNAS 87:4053-4057

| Bradbury, MW and JD Fabricant, 1988. Changes in melanin granules caused by coat color mutations in the fox. J. Hered. 79:133-136

| Chalmers, JH, Jr., MW Bradbury, and JD Fabricant, 1987. A multicolored luminol-based chemiluminescnce demonstration. J. Chem. Ed. 64:969-970

| Bradbury, MW, 1987. Testes of XX ↔ XY chimeric mice develop from fetal ovotestes. Dev. Genet. 8:207-218.

| Bradbury, MW, 1983. Functional capacity of Sex-reversed (XX, Sxr/+) mouse germ cells as shown by progeny derived from a female chimera. J. Exp. Zool. 266:315-320

Books, Chapters, and Reviews:

2015 | Bradbury, MW, K Riggs, E Hengstebeck. Integrating Basic and Clinical Sciences Using an Exercise Aimed at Eliciting Family History from Standardized Patients, IAMSE conference

2015 | Hernandez, M, M Robinson, B Kessler, M Bradbury. Reflections on the First Academic School Year of a New Medical School, IAMSE conference

2015 | Fulton, T, M Bradbury, M Dell, B Dickinson, A Edmondson, S Everse, J Fishback, E Gallman, D Harris, J Jones, V Lyons, J Nixon, A Paganini, A Poznanski, D Russo, J Szarek, A Wilson-Delfosse, L Fall. Unpacking your brain: collaborative identification of core basic science concepts important in clinical decision-making, IAMSE conference

2015 | Bradbury, MW. The role of free fatty acids in the increase in fatty liver disease. Ninety-second Annual Meeting of the Alabama Academy of Science, Inc., Livingston, AL (Oral presentation)

2014 | Bradbury, MW. Implementing active learning at a new medical school and student response to different learning formats, IAMSE conference

2014 | Bradbury, MW. Challenges and Obstacles: ACOM, presented as part of a Focus Session on Challenges and Obstacles for the First Year of a New Medical School at the Annual IAMSE conference (Oral presentation)

2013 | Peterson, HL, A Hudder, MW Bradbury. The effects of excess fructose, glucose, and xylose on lipogenic gene expression and fat accumulation of HuH-7 hepatoma cells in long-term culture. LECOM Research Day

2011 | Ogg, R, DO, S Habusta, N Grimm, M Bradbury. The Effect of Racquet Head Size on Overuse Shoulder Injuries in Competitive Tennis Players. LECOM Research Day

2011 | Bradbury, MW, C Church and R Pratt. Chronic Exposure of HuH7 Hepatoma Cells to Ethanol Causes Increased Fatty Acid Uptake, Which is Reversed by Withdrawal. LECOM Research Day

2011 | Bradbury, MW, S Woodward, R Pratt. Role of Mitochondrial Leader Sequence and Mature Protein Sequence on Subcellular Localization of Mitochondrial Aspartate Aminotransferase. LECOM Research Day

2011 | Evanoff-Jurkovic, M, M Bradbury, J Eysamen, L Epperly. Does pelvic/sacral somatic dysfunction affect the Galleazzi Test? LECOM Research Day, Oral Presentation

2010 | Stefanko, JJ, JL Williams, MW Bradbury, SF Habusta. Direct Pressure Measurements of Vertebroplasty vs Kyphoplasty: Cadaveric Study, LECOM Research Day

2009 | Bradbury, MW, R Pratt. Chronic Ethanol Exposure Reversibly Increases Fatty Acid Uptake in HuH7 Hepatoma Cells. LECOM Research Day

2008 | Bradbury, MW. Identifying Early Indicators of Performance on COMLEX 1, AACOM Meeting

2008 | Dunn, P, R Pratt, MW Bradbury. Assessment of Predicted Structure-Function Relationship in a Fatty Acid Binding Protein, LECOM Research Day

2007 | Pratt, R, E Adewale, MW Bradbury. Targeting a Mitochondrial Enzyme to the Secretory Pathway by Leader Sequence Exchange. LECOM Research Day and TREC Research Day

2006 | Servin-Obert, S, R Pratt and MW Bradbury. Reporter Construct Analysis of the Mitochondrial Aspartate Aminotransferase Promoter. LECOM Research Day

2004 | Berk P, Petrescu O, Bradbury M. Different responses of Osborne-Mendel and S5B/Pl rats to high fat diets, Obesity Research 12, A141-A141

2003 | Petrescu, O, X Fan, MW Bradbury, PD Berk. Selective regulation of long chain fatty acid uptake across adipocyte plasma membranes is a control point for body adiposity. Gastroenterology 124, Suppl 1:A70

2002 | Bradbury, MW, O Petrescu, PD Berk. Identification of mitochondrial aspartate aminotransferase in a population of small vesicles just beneath the plasma membrane in HuH7 cells. Hepatol. 36:239A

2001 | Bradbury, MW, X Fan, DD Stump, JA Odin, F Guarnieri, PD Berk. Mapping the fatty acid binding site in hepatic fatty acid binding protein with molecular mapping and mutagenesis. Hepatol. 34:256A (Oral presentation)

2000 | Bradbury, MW, X Fan, PD Berk. Transcriptional control of the mitochondrial aspartate aminotransferase gene in a model of alcoholic liver disease. Hepatol. 32:331A

2000 | Fan, X, MW Bradbury, DD Stump, PD Berk. Leptin Infusion Downregulates Long Chain Free Fatty Acid Uptake in ob/ob Mouse Adipocytes. Obes. Res. 8, Suppl 1:PA17

2000 | Bradbury, MW, X Fan, PD Berk. Up-regulation of a fatty acid transporter in adipocyte differentiation and alcoholic fatty liver. Devel. Biol. 222:275

1999 | Bradbury, M, S-L Zhou, DD Stump, PD Berk. Differing Effects of a High Fat Diet on Adipocyte Oleic Acid Uptake in S5b/PL and Osborne-Mendel Rats, Physiological Genomics and Rat Models, Cold Spring Harbor.

1999 | Berk, PD, DD Stump, X Fan, MW Bradbury. Cellular uptake of long chain free fatty acids occurs by two distinct processes: Facilitated transport and passive diffusion. Hepatol. 30:462A

1998 | Bradbury, MW, L Delavega, RL Stockert, PD Berk. Green fluorescent protein-tagged mitochondrial aspartate aminotransferase illustrates the intracellular sorting of the native enzyme. Hepatol. 28:511A

1997 | Zhou, S-L, C-L Kiang, M Bradbury, E Albanis, MI Fiel, PD Berk. Increased hepatocellular fatty acid uptake in fatty livers due to EtOH abuse but not those due to obesity. Hepatol. 26:254A

1994 | Zhou, S-L, M Bradbury, C-L Kiang, LM Isola, PD Berk. 3T3 fibroblasts transfected with aspartate aminotransferase cDNAs encoding mutated or deleted mitochondrial leaders fail to exhibit increased fatty acid uptake. Hepatol. 20:174A

1997 | Bradbury, MW, Berk PD. Sequence divergence of mouse mitochondrial aspartate aminotransferase isozymes, 9th International Conference on Genes, Gen Families and Isozymes (Oral presentation)

1996 | Zhou, S-L, R Gordon, C-L Kiang, DD Stump, M Bradbury, LM Isola, PD Berk. Intracellular movement and cellular export of mitochondrial aspartate aminotransferase from hepatoma cell lines. Hepatol. 24:128A

1995 | Zhou, S-L, C-L Kiang, M Bradbury, L Isola, DD Stump, PD Berk. Up-regulation of mitochondrial aspartate aminotransferase mRNA parallels fatty acid uptake in 3T3-L1 cells and adipocytes from obese and diabetic rats. Hepatol. 22:313A

1995 | Zhou, S-L, C-L Kiang, M Bradbury, R Gordon, DD Stump, N-I Han, PD Berk. Effects of EtOH on mitochondrial aspartate aminotransferase expression and cellular leakage and fatty acid uptake in HepG2 cells. Hepatol. 22:241A

1989 | Isola, L, L Glass, MW Bradbury, and J Gordon. Rapid and efficient cloning of DNA flanking foreign genes inserted in transgenic mice using polymerase chain reaction. Blood 74:99A

1989 | Gordon, JW, LM Isola, and MW Bradbury. Rapid determination of sex in preimplantation mouse embryos by blastomere biopsy and enzymatic amplification. Proc. Soc. Gyn. Invest. 36:1

1987 | Fabricant, JD, LC Nuti, BS Minhas, WC Baker, JS Capehart, P Marrack, JH Chalmers, Jr., MW Bradbury, and JE Womack. Gene transfer in goats. Theriogenology 27:229

1984 | White, KL, MW Bradbury, GB Anderson, and RH BonDurant. Immunofluorescent detection of a male-specific factor on preimplantation bovine embryos. Theriogen. 21:275