Timothy P. Geisbuhler, PhD



Work: 660.626.2321
Fax: 660.626.2965
Email Address: tgeisbuhler@atsu.edu

Research Interests: Dr. Geisbuhler's long-term research goal is to understand how metabolic and homeostatic processes in the heart are affected by disease. In recent years, his work has centered on myocardial ischemia. The onset of ischemia triggers a number of metabolic changes in heart cells. One such change is that 5'-nucleotides, the "energy currency" of the cell, are degraded and lost from the tissue. Dr. Geisbuhler uses cells isolated from the heart as a model system to examine strategies for causing rapid resynthesis of nucleotides in postischemic heart. Dr. Geisbuhler also uses isolated cells to study other metabolic phenomena related to ischemic cell injury.

Education:

PhD - Ohio State University (1983)

Scholarly Activity

Research Publications:

2015 | Priest SM [student], Geisbuhler TP. Injectable sodium pentobarbital: Stability at room temperature. J Pharmacol Toxicol Methods. 2015 Jul 31;76:38-42. doi: 10.1016/j.vascn.2015.07.012. [Epub ahead of print]

2013 | Dood KP [Student], Frey AD [Student], Geisbuhler TP (2013) The effect of hawthorn extract on coronary flow. J Evidence-Based Comp Alt Med (JEBCAM) 18: 257-267 (published online ahead of print June 13, 2013: doi:10.1177/2156587213491428).

2009 | Mont MR, Carlson CG, Geisbuhler TP. Resting Ca2+ influx does not contribute to anoxia-induced cell death in adult rat cardiac myocytes. Can J Physiol Pharmacol. 2009 May;87(5):360-70

2008 | Geisbuhler TP. Compartmentalization of non-adenine nucleotides in anoxic cardiac myocytes. Basic Res Cardiol. 2008 Sep 24; [Epub 09-24-07]

2004 | Carnes CA, Geisbuhler TP, Reiser PJ. Age-dependent changes in contraction and regional myocardial myosin heavy chain isoform expression in rats. J Appl Physiol. 2004 Jul;97(1):446-53

2002 | Geisbuhler TP, Schwager TL, Ervin HD. 3-isobutyl-1-methylxanthine (IBMX) sensitizes cardiac myocytes to anoxia. Biochem Pharmacol. 2002 Jun 1;63(11):2055-62

1999 | Geisbuhler TP. Nucleotide dynamics in cardiac cells. Paths to Pyrimidines 1999; 7(1):38-42

1998 | Geisbuhler TP, Schwager TL. Ribose-enhanced synthesis of UTP, CTP, and GTP from parent nucleosides in cardiac myocytes. J Mol Cell Cardiol 1998 Apr;30(4):879-87

1997 | Geisbuhler TP. Anoxia inhibits guanosine salvage in cardiac myocytes. Basic Res Cardiol 1997 Dec;92(6):359-67

1996 | Geisbuhler TP, Schwager TL. Effect of anoxia on cyclic nucleotides and inositol phosphate turnover in cardiac myocytes. J Mol Cell Cardiol 1996 Sep;28(9):1857-66

1995 | Burczynski FJ, Cai Z-S, Moran JB, Geisbuhler T, Rovetto M. Palmitate uptake by cardiac myocytes and endothelial cells. Amer J Physiol 1995 268:H1659-H1666

1992 | Geisbuhler TP, Schwager TL, Rovetto MJ. Guanosine metabolism in adult rat cardiac myocytes: inhibition by acyclovir and analysis of a metabolic pathway. J Mol Cell Cardiol 1992 Jul;24(7):683-90

1991 | Geisbuhler TP, Rovetto MJ. Guanosine metabolism in adult rat cardiac myocytes: ribose-enhanced GTP synthesis from extracellular guanosine. Pflugers Arch 1991 Sep;419(2):160-5

1990 | Geisbuhler TP, Rovetto MJ. Lactate does not enhance anoxia/reoxygenation damage in adult rat cardiac myocytes. J Mol Cell Cardiol 1990 Nov;22(11):1325-35

1987 | Geisbuhler TP, Johnson DA, Rovetto MJ. Cardiac myocyte guanosine transport and metabolism. Am J Physiol 1987 Nov;253(5 Pt 1):C645-51

1987 | Geisbuhler TP, Sergeant S, Miramonti FL, Kim HD, Rovetto MJ. Forskolin inhibition of hexose transport in cardiomyocytes. Pflugers Arch 1987 Jun;409(1-2):158-62

1985 | Lutz WH, Geisbuhler TP, Pollack JD, McClung HJ, Merola AJ. Inhibition of citrulline synthesis by octanoate and its modulation by adenine nucleotides. Biochem Med 1985 Aug;34(1):1-10

1984 | Geisbuhler T, Altchuld RA, Trewyn RW, Ansel AZ, Lamka K, Brierley GP. Adenine nucleotide metabolism and compartmentalization in isolated adult rat heart cells. Circ Res 1984 54: 536-546

1983 | Jurkowitz MS, Geisbuhler T, Jung DW, Brierley GP Ruthenium red-sensitive and –insensitive release of Ca2+ from uncoupled heart mitochondria. Arch Biochem Biophys 1983 223: 120-128

1983 | Geisbuhler TP (Ph.D. Thesis). Distribution and metabolism of adenine nucleotides in rat heart myocytes. Ohio State University. 1983

Presentations and Published Abstracts:

2014 | Priest SM [student], Huber GA [student], Geisbuhler TP*. Hydroxysafflor yellow A and the cardiac permeability transition pore. . Abstract published in: Planta Med. 2014;80(10):850(PU7). Poster presented at the 55th Annual Meeting of the American Society of Pharmacognosy; August 2-6, 2014; Oxford, MS; and at the 3rd Annual Missouri Osteopathic Student and Post-graduate Research Symposium; Missouri Osteopathic Annual Convention; Branson, MO; April 30-May 4, 2014.

2013 | Huber GA [student], Geisbuhler TP. Hydroxysafflor Yellow A Protects Cardiac Myocytes during Anoxia and Reoxygenation. Presented at: American Society of Pharmacognosy, 54th Annual Meeting. Natural Products at a Crossroad - Current and Future Directions. St. Louis, MO. July 13-17, 2013. Published: Planta Med 79: 837 (PH12).

2012 | Keeslar A [student], Brink M [student], Geisbuhler T. The Effect of Cannabinoids on Post-Ischemic Cardiac Performance. Presented at: 33rd Annual Meeting of the International Society for Heart Research – North American Section. Pathology and Treatment of Heart Failure. Banff, AB, Canada, May 28 – 31, 2012. Abstract published in: J Mol Cell Cardiol 53 (#2, supplement): S28 (#95).

2009 | Dood KP [student], Geisbuhler TP; The effect of hawthorn extract on coronary flow; Presented at the 1st Annual Interdisciplinary Biomedical Research Symposium; A.T. Still University of Health Sciences; Kirksville, MO; Sept. 26, 2009. Moscardelli VL, Geisbuhler TP; Hawthorn extract is not cardioprotective in an anoxic cardiomyocyte preparation. FASEB J 21: 2007.

2005 | Mont MR, Geisbuhler TP; Resting Ca2+ influx does not contribute to anoxia-induced cell death in adult rat cardiac myocytes. J Mol Cell Cardiol 37: 863 (#152); 2005.

2004 | Chamberlain NR, Smart JI, Geisbuhler TP. Purification and Characterization of a Bioactive Pheromone from Staphylococcus epidermidis. The 104th General Meeting of the American Society for Microbiology; New Orleans, LA; May 23-27, 2004.

2004 | Theobald RJ, Geisbuhler TP; CB1 Receptor Antagonist SR141716A Effects in Detrusor Smooth Muscle; FASEB J 18: A598; 2004.

2003 | Carlson CG, Geisbuhler TP; Diastolic Calcium Influx in Isolated Rat Cardiac Myocytes; J Mol Cell Cardiol 35: A31 (P-047); 2003.

2002 | Altschuld RA, Carnes CA, Dumitrescu C, Kelley RE, Dech SJ, Geisbuhler TP, Van Wagoner DR; Electromechanical Restitution in Thapsigargin-treated Rat Ventricular Cardiomyocytes; Gordon Research Conference on Cardiac Regulatory Mechanisms; Connecticut College; New London, CT; 2002.

2001 | Geisbuhler TP. 2001. Compartmentalization of Pyrimidine Nucleotides in Anoxic Cardiac Myocytes. J Mol Cell Cardiol 33:A167 (Intl. Soc. for Heart Research, XVII World Congress Satellite Meeting, Regulation of Energy Metabolism in the Heart and Vasculature; Banff, Alberta; July 6-10, 2001).

1999 | Geisbuhler TP, Ervin HD. 1999. Reoxygenation Relieves the Anoxia-Induced Inhibition of Inositol Phosphate Production in Cardiac Myocytes. J Mol Cell Cardiol 31:A11.

1999 | Geisbuhler, Timothy P., Ph.D.: Heart Failure; Presented at Northeast Regional Medical Center, Department of Medical Education; Kirksville, MO; September 21, 1999

1998 | Carlson CG, Geisbuhler TP. 1998. Background Ca2+ Channel Activity in Ventricular Myocytes. University of Missouri 9th Annual Cardiovascular Day Proceedings.

1998 | Geisbuhler TP, Ervin HD. 1998. Anoxia Inhibits Inositol Phosphate Production after Carbachol Challenge in Cardiac Myocytes. Vascular and Myocardial Aspects of Ischemic Heart Disease. Section II:32.

1998 | Geisbuhler TP. Nucleotide Metabolism During Anoxia in Cardiac Myocytes; Ohio University, Dept. Biological Sciences; Athens, OH; April 14, 1998.

1998 | Geisbuhler, Timothy P., Ph.D.: Myocardial Ischemia; Presented at Northeast Regional Medical Center, Department of Medical Education; Kirksville, MO; September 10, 1998

1997 | Geisbuhler TP. 1997. Compartmentation of Guanine Nucleotides in Anoxic Cardiac Myocytes. J Mol Cell Cardiol 29:A195.

1997 | Geisbuhler, Timothy P., Ph.D.: Control of Blood Glucose; Presented at Northeast Regional Medical Center, Department of Medical Education; Kirksville, MO; October 7, 1997

1997 | Geisbuhler, Timothy P., Ph.D.: Metabolic Effects of Myocardial Ischemia; Presented at Northeast Regional Medical Center, Department of Medical Education; Kirksville, MO; September 11, 1997

1997 | Geisbuhler, Timothy P., Ph.D.: Metabolic Effects of Myocardial Ischemia; Presented at Northeast Regional Medical Center, Department of Medical Education; Kirksville, MO; January 7, 1997