Keith S. Elmslie, PhD

Chair

Work: 660.626.2384
Fax: 660.626.2728
Email Address: kelmslie@atsu.edu

Research Interests: 1. Determinants of ω-conotoxin binding to the N-type calcium channel. 2. Molecular determinants of calcium channel gating. 3. Effects of ceramide on ion channel gating. 4. Cerebrospinal fluid proteins as markers for neuropathic pain. 5. Determining the effect of intravenous anesthetics on L-type calcium channels in human atrial myocytes. 6. Identification of novel tri-substituted purines that specifically affect ion channel gating. 7. Mechanisms controlling excitability of the exercise pressor reflex.

Education:

Postdoctoral Fellow - Case Western Reserve University (1991)
PhD - (Biology) State University of New York, Stony Brook (1988)
MS - (Biology) University of Utah (1983)
BS - (Biology & Chemistry) Westminster College (1980)

Scholarly Activity

Research Publications:

2016 | Ramachandra R [staff], Elmslie KS. EXPRESS: Voltage-dependent sodium (NaV) channels in group IV sensory afferents. Mol Pain. 2016 Jul 5;12. pii: 1744806916660721. doi: 10.1177/1744806916660721. Print 2016

2015 | Luo S, Zhangsun D, Harvey PJ, Kaas Q, Wu Y, Zhu X, Hu Y, Li X, Tsetlin VI, Christensen S, Romero HK, McIntyre M, Dowell C, Baxter JC [student], Elmslie KS, Craik DJ, McIntosh JM. Cloning, synthesis, and characterization of αO-conotoxin GeXIVA, a potent α9α10 nicotinic acetylcholine receptor antagonist. Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):E4026-35. doi: 10.1073/pnas.1503617112. Epub 2015 Jul 13.

2015 | Wright AB [student], Norimatsu Y, McIntosh JM, Elmslie KS. Limited efficacy of α-conopeptides, Vc1.1 and RgIA, to inhibit sensory neuron CaV current. eNeuro. 2015;2(1).

2015 | Buraei Z, Lee HK, Elmslie KS. Single channel measurements demonstrate the voltage dependence of permeation through N-type and L-type CaV channels. Channels (Austin). 2015 Jan 2;9(1):50-5. doi: 10.4161/19336950.2014.991606.

2014 | Buraei Z, Liang H, Elmslie KS. Voltage control of Ca2+ permeation through N-type calcium (CaV2.2) channels. J Gen Physiol. 2014 Sep;144(3):207-20. doi:10.1085/jgp.201411201. Epub 2014 Aug 11

2014 | Baxter JC [student], Ramachandra R [post-doc], Mayne DR [sstudent], Elmslie KS. Functional Expression of α7 Nicotinic Acetylcholine Receptors by Muscle Afferent Neurons. J Neurophysiol. 2014 Jun 25; 112:1549-1558. pii: jn.00035.2014. [Epub ahead of print]

2013 | Ramachandra R [post-doc], McGrew S, Elmslie K. Identification of specific sensory neuron populations for study of expressed ion channels. J Vis Exp. 2013 Dec 24;(82):50782. doi: 10.3791/50782

2013 | Ramachandra R [post-doc], Hassan B, McGrew SG, Dompor J [student], Farrag M, Ruiz-Velasco V, Elmslie KS. Identification of CaV channel types expressed in muscle afferent neurons.J Neurophysiol. 2013 Oct;110(7):1535-43. doi: 10.1152/jn.00069.2013. Epub 2013 Jul 10.

2012 | Ramachandra R [post-doc], McGrew SY, Baxter JC [student], Howard JR [student], Elmslie KS. NaV1.8 channels are expressed in large, as well as small, diameter sensory afferent neurons. Channels (Austin). 2013 Jan 1;7(1):34-7. doi: 10.4161/chan.22445. Epub 2012 Oct 12.

2012 | Ramachandra R [post-doc], McGrew SY, Baxter JC [student],, Kiveric E [Student],, Elmslie KS. Tetrodotoxin-resistant voltage-dependent sodium channels in identified muscle afferent neurons. J Neurophysiol. 2012 Oct;108(8):2230-41. doi: 10.1152/jn.00219.2012. Epub 2012 Aug 1

2012 | Gowd KH, Blais KD [student],, Elmslie KS, Steiner AM, Olivera BM, Bulaj G. Dissecting a role of evolutionary-conserved but noncritical disulfide bridges in cysteine-rich peptides using ω-conotoxin GVIA and its selenocysteine analogs. Biopolymers. 2012;98(3):212-23. doi: 10.1002/bip.22047

2011 | Yarotskyy V, Gao G, Peterson BZ, Elmslie KS. Domain III regulates N-type (CaV2.2) calcium channel closing kinetics. J Neurophysiol. 2012 Apr;107(7):1942-51. Epub 2011 Dec 28

2012 | Yarotskyy V, Elmslie KS. Roscovitine inhibits CaV3.1 (T-type) channels by preferentially affecting closed-state inactivation. J Pharmacol Exp Ther. 2012 Feb;340(2):463-72. Epub 2011 Nov 16.

2010 | Fassl J, High KM, Stephenson ER, Yarotskyy V, Elmslie KS. The intravenous anesthetic propofol inhibits human L-Type calcium channels by enhancing voltage-dependent inactivation. J Clin Pharmacol. 2011 May;51(5):719-30; 2010 Jun 14. [Epub ahead of print]

2010 | Yarotskyy V, Elmslie KS. Interference between two modulators of N-type (CaV2.2) calcium channel gating demonstrates that omega-conotoxin GVIA disrupts open state gating. Biochim Biophys Acta. 2010 Sep;1798(9):1821-1828. Epub 2010 May 13.

2010 | Ganapathi SB, Fox TE, Kester M, Elmslie KS. Ceramide modulates HERG potassium channel gating by translocation into lipid rafts. Am J Physiol Cell Physiol. 2010 Jul;299(1):C74-86. Epub 2010 Apr 7

2010 | Guo K, Wang X, Gao G, Huang C, Elmslie KS, Peterson BZ. Amino acid substitutions in the FXYD motif enhance phospholemman-induced modulation of cardiac L-type calcium channels. Am J Physiol Cell Physiol. 2010 Nov;299(5):C1203-11. Epub 2010 Aug 18

2010 | Wang X, Gao G, Guo K, Yarotskyy V, Huang C, Elmslie KS, Peterson BZ. Phospholemman modulates the gating of cardiac L-type calcium channels. Biophys J. 2010 Apr 7;98(7):1149-59

2010 | Gowd KH, Yarotskyy V, Elmslie KS, Skalicky JJ, Olivera BM, Bulaj G. Site-specific effects of diselenide bridges on the oxidative folding of a cystine knot peptide, omega-selenoconotoxin GVIA. Biochemistry. 2010 Mar 30;49(12):2741-52.

2010 | Li Z, Wang X, Gao G, Qu D, Yu B, Huang C, Elmslie KS, Peterson BZ. A single amino acid change in Ca(v)1.2 channels eliminates the permeation and gating differences between Ca(2+) and Ba(2+). J Membr Biol. 2010 Feb;233(1-3):23-33. Epub 2010 Jan 23

2010 | Yarotskyy V, Gao G, Du L, Ganapathi SB, Peterson BZ, Elmslie KS. Roscovitine binds to novel L-channel (CaV1.2) sites that separately affect activation and inactivation. J Biol Chem. 2010 Jan 1;285(1):43-53

2009 | Yarotskyy V, Elmslie KS. Open-state occupancy prevents gating charge relaxation of N-type (CaV2.2) calcium channels. Biophys J. 2009 Nov 4;97(9):2446-55

2009 | Ganapathi SB, Kester M, Elmslie KS. State-dependent block of HERG potassium channels by R-roscovitine: implications for cancer therapy. Am J Physiol Cell Physiol. 2009 Apr;296(4):C701-10

2009 | Yarotskyy V, Gao G, Peterson BZ, Elmslie KS. The Timothy syndrome mutation of cardiac CaV1.2 (L-type) channels: multiple altered gating mechanisms and pharmacological restoration of inactivation. J Physiol. 2009 Feb 1;587(Pt 3):551-65.

2009 | Yarotskyy V, Elmslie KS. omega-conotoxin GVIA alters gating charge movement of N-type (CaV2.2) calcium channels. J Neurophysiol. 2009 Jan;101(1):332-40.

2008 | Buraei Z, Elmslie KS. The separation of antagonist from agonist effects of trisubstituted purines on CaV2.2 (N-type) channels. J Neurochem. 2008 May;105(4):1450-61

2007 | Yarotskyy V, Elmslie KS. Roscovitine, a cyclin-dependent kinase inhibitor, affects several gating mechanisms to inhibit cardiac L-type (Ca(V)1.2) calcium channels. Br J Pharmacol. 2007 Oct;152(3):386-95

2007 | Buraei Z, Schofield G, Elmslie KS. Roscovitine differentially affects CaV2 and Kv channels by binding to the open state. Neuropharmacology. 2007 Mar;52(3):883-94

2006 | Goo YS, Lim W, Elmslie KS. Ca2+ enhances U-type inactivation of N-type (CaV2.2) calcium current in rat sympathetic neurons. J Neurophysiol. 2006 Sep;96(3):1075-83

2005 | Buraei Z, Anghelescu M, Elmslie KS. Slowed N-type calcium channel (CaV2.2) deactivation by the cyclin-dependent kinase inhibitor roscovitine. Biophys J. 2005 Sep;89(3):1681-91

2003 | Elmslie KS. Neurotransmitter modulation of neuronal calcium channels. J Bioenerg Biomembr. 2003 Dec;35(6):477-89

2004 | Elmslie KS. Calcium channel blockers in the treatment of disease. J Neurosci Res. 2004 Mar 15;75(6):733-41

2002 | Liang H, Elmslie KS. Rapid and reversible block of N-type calcium channels (CaV 2.2) by omega-conotoxin GVIA in the absence of divalent cations. J Neurosci. 2002 Oct 15;22(20):8884-90.

2001 | Liang H, Elmslie KS. E(f)-current contributes to whole-cell calcium current in low calcium in frog sympathetic neurons. J Neurophysiol. 2001 Sep;86(3):1156-63.

2000 | Elmslie KS. N-type Calcium Channels. Korean J. Physiol. Pharmacol. 2000. 4:427-437

2000 | Goo YS, Elmslie KS. Role of protein kinase C on norepinephrine induced inhibition of calcium current in rat sympathetic neurons. Kor. J. Med. Physics. 2000. 11:29-38

2000 | Lee HK, Elmslie KS. Reluctant gating of single N-type calcium channels during neurotransmitter-induced inhibition in bullfrog sympathetic neurons. J Neurosci. 2000 May 1;20(9):3115-28

2000 | Lee HK, Liu L, Elmslie KS. Effect of high Ba(2+) on norepinephrine-induced inhibition of N-type calcium current in bullfrog sympathetic neurons. J Neurophysiol. 2000 Feb;83(2):791-5

1999 | Lee HK, Elmslie KS. Gating of single N-type calcium channels recorded from bullfrog sympathetic neurons. J Gen Physiol. 1999 Jan;113(1):111-24. PubMed PMID: 9874692

1997 | Elmslie KS. Identification of the single channels that underlie the N-type and L-type calcium currents in bullfrog sympathetic neurons. J Neurosci. 1997 Apr 15;17(8):2658-68

1997 | Jones SW, Elmslie KS. Transmitter modulation of neuronal calcium channels. J Membr Biol. 1997 Jan 1;155(1):1-10. Review

1995 | Ehrlich I, Elmslie KS. Neurotransmitters acting via different G proteins inhibit N-type calcium current by an identical mechanism in rat sympathetic neurons. J Neurophysiol. 1995 Dec;74(6):2251-7.

1994 | Elmslie KS, Jones SW. Concentration dependence of neurotransmitter effects on calcium current kinetics in frog sympathetic neurones. J Physiol. 1994 Nov 15;481 ( Pt 1):35-46

1994 | Elmslie KS, Kammermeier PJ, Jones SW. Reevaluation of Ca2+ channel types and their modulation in bullfrog sympathetic neurons. Neuron. 1994 Jul;13(1):217-28.

1993 | Werz MA, Elmslie KS, Jones SW. Phosphorylation enhances inactivation of N-type calcium channel current in bullfrog sympathetic neurons. Pflugers Arch. 1993 Sep;424(5-6):538-45

1993 | van Lunteren E, Elmslie KS, Jones SW. Effects of temperature on calcium current of bullfrog sympathetic neurons. J Physiol. 1993 Jul;466:81-93

1993 | Elmslie KS, Werz MA, Overholt JL, Jones SW. Intracellular ATP and GTP are both required to preserve modulation of N-type calcium channel current by norepinephrine. Pflugers Arch. 1993 Jun;423(5-6):472-9

1992 | Elmslie KS, Kammermeier PJ, Jones SW. Calcium current modulation in frog sympathetic neurones: L-current is relatively insensitive to neurotransmitters. J Physiol. 1992 Oct;456:107-23

1992 | Elmslie KS. Calcium current modulation in frog sympathetic neurones: multiple neurotransmitters and G proteins. J Physiol. 1992;451:229-46

1992 | Jones SW, Elmslie KS. Separation and modulation of calcium currents in bullfrog sympathetic neurons. Can J Physiol Pharmacol. 1992;70 Suppl:S56-63. Review.

1990 | Elmslie KS, Zhou W, Jones SW. LHRH and GTP-gamma-S modify calcium current activation in bullfrog sympathetic neurons. Neuron. 1990 Jul;5(1):75-80

1990 | Elmslie KS, Cohen DH. Iontophoresis of norepinephrine onto neurons of the pigeon's lateral geniculate nucleus: characterization of an inhibitory response. Brain Res. 1990 May 28;517(1-2):134-42.

1985 | Elmslie KS, Yoshikami D. Effects of kynurenate on root potentials evoked by synaptic activity and amino acids in the frog spinal cord. Brain Res. 1985 Mar 25;330(2):265-72.

2017 | Romero HK, Christensen SB, Di Cesare Mannelli L, Gajewiak J, Ramachandra R, Elmslie KS, Vetter DE, Ghelardini C, Iadonato SP, Mercado JL, Olivera BM, McIntosh JM. Inhibition of α9α10 nicotinic acetylcholine receptors prevents chemotherapy-induced neuropathic pain. Proc Natl Acad Sci U S A. 2017 Feb 21. pii: 201621433. doi: 10.1073/pnas.1621433114. [Epub ahead of print] PubMed

Books, Chapters, and Reviews:

2005 | Elmslie KS. Passive Propagation of Electrical Signals. Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester. http://www.els.net/ [doi:10.1038/npg.els.0000197] 2005

2000 | Elmslie KS. Membrane Potential. Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester. http://www.els.net/ [doi:10.1038/npg.els.0000182] 2000

2000 | Elmslie KS. Action Potential: Ionic Mechanisms. Encyclopedia of Life Sciences. John Wiley & Sons, Ltd: Chichester. http://www.els.net/ [doi:10.1038/npg.els.0000002] 2000.

Presentations and Published Abstracts:

2016 | Ramachandra R, Elmslie KS. Using specific blockers to identify TTX-S NaV channels subtypes in Rat muscle afferent neurons. Poster presented at the 8th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 5, 2016.

2015 | Ramachandra R [post-doc], Elmslie KL, Koirala A, Elmslie KS. NaV Channels Expressed in Muscle Afferent Neurons and Axons. Poster presented at the 7th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 31, 2015.

2015 | Wright AB [student]*, Ramachandra R [post-doc], Elmslie KS. KV7 channels are potential regulators of the exercise pressor reflex. Poster presented at: Experimental Biology 2015; March 28-April 1, 2015; Boston, MA. Abstract published in: FASEB J. 2015;29(1 suppl):837.4.

2014 | Marler TL [student], Elmslie KS. Expression of NaV1.9 Channels by Muscle Afferent Neurons. Poster presented at the Experimental Biology annual meeting; San Diego, CA; April 29, 2014. Abstract published in: FASEB J. 2014;28(1 suppl):1129.10.

2014 | Ramachandra R [post-doc], Elmslie KS. Ion Channels and Receptors in Group IV Muscle Afferents. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2014 | Ramachandra R* [post-doc], Elmslie KS. Ion Channels/Receptors Expressed along Group IV Sensory Afferent Fibers within Rat Skeletal Muscle. Presented at: Society for Neuroscience 2014 Annual Meeting; Washington, DC; November 15-19, 2014.

2014 | Wright AB [student], Ramachandra R [post-doc], Elmslie KS. KV7 Channels are Potential Regulators of the Exercise Pressor Reflex. Poster presented at 6th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 1, 2014.

2013 | Baxter JC [student], Elmslie KS. Nicotinic Acetylcholine Receptors Expressed by Muscle Afferent Neurons. Presented at: Experimental Biology 2013; Boston, MA; April 22, 2013.

2013 | Ramachandra R, Elmslie KS (2013) Kv currents in group III/ IV muscle afferent neurons. Abstract #422.10. Annual meeting of the Society for Neuroscience; San Diego, CA; Nov. 9-13, 2013.

2013 | Ramachandra R, Elmslie KS. KV Currents in Identified Muscle Afferents. Poster presented at 5th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 2, 2013.

2013 | Wright A [student], Baxter JC [student], Elmslie KS. The Analgesic Effect of α9/10 Toxins Does Not Involve Activation of GABAB Receptors. Poster presented at 5th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; November 2, 2013.

2012 | Baxter J [student], Elmslie KS. Chemical Activators of Muscle Afferent Neurons that Generate the Exercise Pressor Reflex. Poster presented at 4th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; September 29, 2012.

2012 | Ramachandra R [post-doc], Hassan B, Ruiz-Velasco V, Elmslie KS. Functional Identification of CaV Channel Types Expressed in Group III/IV Muscle Afferent Neurons. Abstract # 235.18; 2012. Society for Neuroscience 2012; New Orleans, LA; October 13-17, 2012.

2012 | Ramachandra R [post-doc], McGrew SY [staff], Elmslie KS. Functional Identification of CaV Channel Types Expressed in Muscle Afferent Neurons. Poster presented at 4th Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; September 29, 2012.

2011 | Ramachandra R [post-doc], McGrew S [staff], Baxter J [student], Elmslie KS. Pharmacological Identification of Voltage-gated Sodium Channels Expressed in Muscle Afferent Neurons. Poster presented at 3rd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 22, 2011.

2011 | Yarotskyy V, Ramachandra R, Elmslie KS. ω-Conotoxins Mviia and mviic Differenti ally Affect N-type Calcium Channel Gating. Presented at: Venoms to Drugs; Heron Island, Queensland, Australia; May 16, 2011

2010 | Ramachandra R [post-doc], McGrew S, Blais K [student], Yaroskyy V, Elmslie KS. Voltage-dependent Sodium Channels that Mediate Excitability of Muscle Afferents that Generate the Exercise Pressor Reflex. Poster presented at 2nd Annual Interdisciplinary Biomedical Research Conference; Kirksville, MO; October 9, 2010.

2010 | Yarotskyy V, Elmslie KS. Interference Between Two Modulators of N-type (CaV2.2) Calcium Channel Gating Charge Movement. Presented at: Biophysical Society 54th Annual Meeting; San Francisco, CA; February 20-24, 2010